Antibiotic Dilution Calculator

Calculate the volume of stock antibiotic needed to prepare a working solution.

Aug 31, 2026 Microbiology #microbiology #antibiotic-dilution #c1v1 #antimicrobial

Calculate

All fields required unless noted

Stock concentration (µg/mL)

Desired concentration (µg/mL)

Desired final volume (mL)

Calculating...

Enter your values and press Calculate to see the result.

Formula & Clinical Notes

Formula

C1V1 = C2V2

Clinical Notes

Using C1V1 = C2V2: stock volume = (desired concentration × desired volume) / stock concentration; the remainder is diluent. The desired concentration cannot exceed the stock concentration. Always account for the final volume in your medium.

Sources

CLSI M07 — Methods for Dilution Antimicrobial Susceptibility Tests