Purpose
To provide a standardized framework for performing Albert Staining.
Scope
This procedure applies to trained microbiology laboratory personnel working within an approved quality-management and biosafety system.
Principle
Albert staining differentially demonstrates cellular morphology and characteristic granules in organisms for which the method is validated.
Specimen / Test Material
Suitable smears prepared from relevant clinical isolates or specimens.
Equipment and Materials
- Clean slides
- Validated Albert stain reagents
- Microscope
- Appropriate control material
Procedure
- Prepare a suitable smear.
- Fix according to the validated procedure.
- Apply Albert staining reagents as specified by the approved method.
- Examine microscopically.
- Record morphology and relevant staining characteristics.
Quality Control and Quality Assurance
- Use known control material where required.
- Reject poor-quality smears or staining reactions.
Reporting
Report the staining reaction and relevant microscopic findings using approved terminology.
Safety
Use risk-based biosafety practices, appropriate personal protective equipment and approved decontamination and waste-disposal procedures. Treat clinical specimens and cultures according to the laboratory's biological risk assessment.
References
- World Health Organization (WHO): Toolkit for Standard Operating Procedures for Bacteriology, Mycology and Antimicrobial Susceptibility Testing.
- WHO: Laboratory Biosafety Manual, 4th edition.
- WHO guidance on collection, packaging and transport of infectious substances.
- EUCAST methodology and quality-control guidance for antimicrobial susceptibility testing, where applicable.
- Current locally approved laboratory procedures and validated manufacturer instructions.