Purpose
To separate and identify haemoglobin fractions using a validated electrophoretic method.
Principle
Haemoglobin fractions migrate differently in an electrical field depending on their physical and chemical properties under defined analytical conditions.
Specimen
Use a properly collected and correctly identified specimen suitable for the validated electrophoretic method.
Equipment and Materials
- Validated electrophoresis system
- Approved separation medium and buffers
- Power supply or automated analyser
- Appropriate controls
- Personal protective equipment
Procedure
- Verify specimen identification and suitability.
- Prepare the sample according to the validated method.
- Prepare the electrophoretic system according to the approved procedure.
- Apply specimens and controls.
- Perform separation under validated conditions.
- Verify acceptable control performance.
- Interpret migration patterns using approved criteria.
- Report findings according to laboratory policy.
Quality Control
Run appropriate controls and verify acceptable separation before releasing patient results.
Interpretation
Interpretation depends on the analytical method and the pattern obtained. Some haemoglobin variants may require additional or confirmatory methods.
Safety
Follow electrical safety procedures and standard precautions for biological specimens.