Standard Operating Procedures
Laboratory SOPs
Standard operating procedures for laboratory professionals, from specimen collection to instrument quality control.
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Serial Dilution Technique
General laboratory procedure for producing sequential dilutions using a defined dilution factor.
Manual Complete Blood Count (CBC) Procedure
Standard Operating Procedure (SOP) for performing a manual Complete Blood Count (CBC), including WBC, RBC, Platelet, Hemoglobin, PCV, and differential counts using a hemocytometer and microscopy.
Erythrocyte Sedimentation Rate (Westergren Method)
SOP for measuring the rate at which red blood cells settle in a vertical tube over one hour, indicating non-specific inflammation.
D-Dimer Testing Procedure
Standard Operating Procedure (SOP) for quantitative and semi-quantitative D-dimer testing using immunoassay methods for the evaluation of thrombotic disorders.
Urine Microscopy (Sediment Examination)
SOP for the microscopic examination of centrifuged urine sediment to identify formed elements like cells, casts, crystals, and microorganisms.
Activated Partial Thromboplastin Time (APTT)
SOP for evaluating the intrinsic and common coagulation pathways and monitoring unfractionated heparin therapy.
Prothrombin Time (PT) and INR
SOP for evaluating the extrinsic and common coagulation pathways and monitoring oral anticoagulant therapy.
Packed Cell Volume (Microhematocrit)
SOP for determining the percentage of whole blood volume occupied by red blood cells using centrifugation.
Manual Reticulocyte Count Procedure
Standard Operating Procedure (SOP) for performing a manual reticulocyte count using supravital staining and light microscopy to assess bone marrow erythropoietic activity.
Hanging Drop Method for Bacterial Motility
SOP for hanging drop preparation to observe bacterial motility and true motility vs. Brownian movement.
McFarland Standard Preparation and Use
SOP for preparation and use of McFarland turbidity standards for standardizing bacterial inoculum density.
Kirby-Bauer Disc Diffusion Antimicrobial Susceptibility Testing
SOP for standardized disc diffusion method to determine bacterial susceptibility to antimicrobial agents.
Gram Staining Procedure
SOP for differential Gram staining to classify bacteria as Gram-positive or Gram-negative and assess morphology.
Urinalysis (Chemical Reagent Strip)
SOP for the chemical analysis of urine using a multi-parameter reagent strip to detect metabolic, renal, and systemic abnormalities.
Thick Blood Film Preparation and Examination
SOP for preparing and examining thick blood films, primarily for the detection of malaria parasites and other blood-borne pathogens.
Peripheral Blood Film Examination and Morphology Assessment
Standard Operating Procedure (SOP) for the preparation, staining, microscopic examination, and morphological assessment of peripheral blood films for diagnostic hematology.