Purpose
To screen for the possible presence of haemoglobin S using a validated solubility method.
Principle
Under defined reducing conditions, haemoglobin S demonstrates reduced solubility, producing a characteristic analytical reaction compared with an appropriate negative sample.
Specimen
Use a specimen suitable for the laboratory's validated haemoglobin S screening method.
Equipment and Materials
- Validated solubility-test reagent system
- Appropriate reaction tubes or assay device
- Quality-control materials where required
- Personal protective equipment
Procedure
- Verify specimen identification and suitability.
- Prepare the test reagent according to the validated method.
- Add the specimen as specified by the approved procedure.
- Allow the reaction to proceed under validated conditions.
- Assess the reaction according to the method's interpretation criteria.
- Record the result.
Quality Control
Use appropriate positive and negative controls according to the laboratory's validated procedure.
Interpretation
The test is a screening procedure and does not independently establish haemoglobin genotype.
Safety
Follow laboratory safety procedures for blood specimens and reagents.