Calculator
Bacterial Concentration (OD600) Calculator
Estimate bacterial concentration from optical density at 600 nm.
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All fields required unless notedEnter your values and press Calculate to see the result.
Formula & Clinical Notes
Formula
CFU/mL = OD600 × Conversion factor
Clinical Notes
Concentration = OD600 × conversion factor. Typical factors are about 1 × 10⁸ CFU/mL per OD unit for E. coli grown in LB medium, but this varies with organism, strain, growth phase and spectrophotometer. Calibrate the factor for your own system for accurate results.
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