Standard Operating Procedures
Laboratory SOPs
Standard operating procedures for laboratory professionals, from specimen collection to instrument quality control.
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Optochin Susceptibility Test
Phenotypic susceptibility screening test using optochin under standardized conditions as part of a validated identification workflow.
Novobiocin Susceptibility Test
Phenotypic susceptibility screening test using novobiocin under standardized conditions as part of a validated identification workflow.
Bile Esculin Test
Biochemical identification procedure for bile esculin test using a validated test system.
SIM Test
Biochemical identification procedure for sim test using a validated test system.
Voges-Proskauer Test
Biochemical identification procedure for voges-proskauer test using a validated test system.
Methyl Red Test
Biochemical identification procedure for methyl red test using a validated test system.
Triple Sugar Iron (TSI) Test
Biochemical identification procedure for triple sugar iron (tsi) test using a validated test system.
Urease Test
Biochemical identification procedure for urease test using a validated test system.
Citrate Utilization Test
Biochemical identification procedure for citrate utilization test using a validated test system.
Indole Test
Biochemical identification procedure for indole test using a validated test system.
Oxidase Test for Bacterial Identification
SOP for detecting cytochrome c oxidase to differentiate bacterial groups (e.g., Pseudomonas vs. Enterobacteriaceae).
Coagulase Test for Staphylococcal Identification
SOP for detecting coagulase enzyme to differentiate Staphylococcus aureus from other staphylococci.
Catalase Test for Bacterial Identification
SOP for catalase test to differentiate bacteria based on production of catalase enzyme.
Hanging Drop Method for Bacterial Motility
SOP for hanging drop preparation to observe bacterial motility and true motility vs. Brownian movement.
Kirby-Bauer Disc Diffusion Antimicrobial Susceptibility Testing
SOP for standardized disc diffusion method to determine bacterial susceptibility to antimicrobial agents.
Bacterial Culture and Antimicrobial Susceptibility Testing
SOP for bacterial culture, isolation, identification, and antimicrobial susceptibility testing (AST).
Ziehl-Neelsen Staining for Acid-Fast Bacilli
SOP for Ziehl-Neelsen staining to detect acid-fast bacilli (Mycobacterium tuberculosis and other mycobacteria).
Gram Staining Procedure
SOP for differential Gram staining to classify bacteria as Gram-positive or Gram-negative and assess morphology.
Urine Culture and Colony Count
SOP for quantitative urine culture to diagnose urinary tract infections (UTI) and identify causative organisms.